ERNIE-ac4C: A Novel Deep Learning Model for Effectively Predicting N4-acetylcytidine Sites

Abstract

RNA modifications are known to play a critical role in gene regulation and cellular processes. Specifically, N4-acetylcytidine (ac4C) modification has emerged as a significant marker involved in mRNA translation efficiency, stability, and various diseases. Accurate identification of ac4C modification sites is essential for unraveling its functional implications. However, currently available experimental methods suffer from drawbacks such as lengthy detection times, complexity, and high costs, resulting in low efficiency and accuracy in prediction. Although several bioinformatics methods have been proposed and have advanced the prediction of ac4C modification sites, there is still ample room for improvement. In this research, we propose a novel deep learning model, ERNIE-ac4C, which combines the ERNIE-RNA language model and a two-dimensional Convolutional Neural Network (CNN). ERNIE-ac4C utilizes the fusion of sequence features and attention map features to predict ac4C modification sites. ERNIE-ac4C surpasses other state-of-the-art deep learning methods, demonstrating superior accuracy and effectiveness. The availability of the code on GitHub (https://github.com/lrlbcxdd/ERNIEac4C.git) and our openness to feedback from the research community contribute to the model's accessibility and its potential for further advancements. Our study provides valuable insights into ac4C research and enhances our understanding of the functional consequences of RNA modifications. © 2025 Elsevier Ltd