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초록

Abnormal levels of trypsin in the human body can lead to various diseases, yet conventional detection methods often lack operational simplicity and real-time readout capabilities. This work presents a state-of-the-art metal organic framework (MOF) nanozyme-integrated liquid crystal (LC) sensor (MHN-LC sensor) and demonstrates the detection of trypsin as a proof of the concept. By rational engineering of the MOF-808 framework with Al3+ and l-histidine coordination, a novel MOF nanozyme (MHis-NE) exhibiting exceptional acetylcholinesterase (AChE)-mimetic activity is successfully prepared. In the presence of trypsin, the gelatin hydrogel-encapsulated MHis-NE immobilized on the centrifuge tube lid is released into the aqueous solution due to trypsin-triggered gelatin degradation. Subsequently, a surfactant myristoylcholine is catalytically hydrolyzed by released MHis-NE into choline and myristic acid. This enzymatic cascade induces a characteristic LC optical transition from a dark to bright appearance, corresponding to the interfacial LC molecular reorientation from homeotropic to planar alignment. This MHis-NE LC sensor achieves a detection limit of 1.09 x 10-5 mg/mL for trypsin with a broad detection range from 10-4 to 1 mg/mL. It also can be applied to trypsin quantification in human serum with superior selectivity against other enzymes as potential interferents. The developed strategy not only advances the nanozyme-mediated signal transduction principle but also significantly expands the potential of various LC sensors.