ScholarWorks@성균관대학교

초록

A novel bacterial strain, designated MF1-12-2T, was isolated from tidal mud collected at the Gochang coast, Republic of Korea. Cells of strain MF1-12-2T were non-motile, Gram-stain-negative short rods and formed pale white, circular colonies on marine agar after 6 days of incubation at 25 degrees C. The strain grew optimally at 25 degrees C, pH 7.0, and in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on the 16 S rRNA gene sequence showed that strain MF1-12-2T belongs to the genus Silicimonas within the family Roseobacteraceae, with the highest sequence similarity (97.0%) to Silicimonas algicola DSM 103,371T. The genome of strain MF1-12-2T is 4.3 Mbp, with a DNA G + C content of 60.8%. The ANI (72.6%), AAI (72.6%), and digital DNA-DNA hybridization (dDDH; 19.3%) values between strain MF1-12-2(T) and Silicimonas algicola DSM 103371(T), further support its status as a novel species. The major fatty acids were C18:0, C18:1 omega 7c, and C18:1 omega 7c 11-methyl. The predominant polar lipids were phosphatidylcholine (PC), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG). The respiratory quinone was ubiquinone-10 (Q-10). Based on its polyphasic taxonomic characteristics, strain MF1-12-2(T) represents a novel species within the genus Silicimonas, for which the name Silicimonas lutimaris sp. nov. is proposed. The type strain is MF1-12-2T (= KEMB 24353T =KCTC 8886T =TBRC 21243T).

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