ScholarWorks@성균관대학교

초록

A Gram-negative, aerobic, non-motile, ovoid bacterium, designated strain MF3-039T, was isolated from tidal mudflat sediment sampled in Gunsan, Republic of Korea. The strain formed yellow-pigmented colonies on marine agar and was positive for catalase and oxidase. It grew at temperatures ranging from 10 to 37 degrees C (optimum, 25 degrees C), pH levels from 6.0 to 9.0 (optimum, 7.0), and 0 to 7.0% (w/v) NaCl (optimum, 2.0%). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain MF3-039T clustered within the genus Altererythrobacter (family Erythrobacteraceae), with closest sequence similarity to Altererythrobacter insulae KCTC 63421T (97.9%), Altererythrobacter ishigakiensis ATCC BAA-2084T (97.9%), Altererythrobacter rubellus NBRC 112769T (97.2%), and Tsuneonella suprasediminis KCTC 62388T (97.1%). The draft genome was 2.9 Mbp with a G + C content of 60%. Genomic comparisons indicated average nucleotide identity and digital DNA-DNA hybridisation values below 95% and 70%, respectively, with related Altererythrobacter species. Chemotaxonomic analysis revealed ubiquinone-10 as the primary respiratory quinone, C17:1 omega 6c and summed feature 8 (comprising C18:1 omega 7c/C18:1 omega 6c), and summed feature 3 (C16:1 omega 7c/C16:1 omega 6c) as predominant fatty acids, and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified glycolipid, and an unidentified lipid as major polar lipids. The combination of phenotypic, chemotaxonomic, genomic, and phylogenetic features clearly distinguishes strain MF3-039T from existing genera within the family Erythrobacteraceae. On the basis of these polyphasic analyses, strain MF3-039T represents a novel genus and species, for which the name Litorerythrobacter xanthomarinus gen. nov., sp. nov. is proposed. The type strain is MF3-039T (= KEMB 23948T = KCTC 8705T = TBRC 19385T).

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