ScholarWorks@성균관대학교

초록

Monitoring disease-related biomarkers, such as uric acid in human body fluids, is essential for effective disease management and clinical diagnosis. In this study, copper-coordinated nitrogen-doped carbon dots (Cu@N-CDs) were synthesized via a simple hydrothermal method, achieving a remarkable photoluminescence quantum yield of 44.69%. The Cu@N-CDs emitted fluorescence at 460 nm upon excitation at 360 nm, making them highly suitable for sensitive biosensing applications. For uric acid detection, a Cu@N-CDs-based probe was developed and coupled with phenol (ph-OH) and 4-aminoantipyrine (AP-NH2). In the presence of hydrogen peroxide (H2O2), generated through the enzymatic breakdown of uric acid by uricase, ph-OH and AP-NH2 reacted to form a pink-colored compound. This compound quenched the fluorescence emission of Cu@N-CDs via an inner-filter effect, enabling fluorometric detection. Additionally, for colorimetric detection, the pink compound was quantified by measuring absorbance at 510 nm. The detection strategy utilized the peroxidase-mimetic activity of Cu@N-CDs, which was further enhanced by the presence of Cu. Under optimized conditions, the fluorometric method demonstrated a linear detection range of 0.01-700 mu M, while the colorimetric method showed a range of 0.07-700 mu M for uric acid. The developed approach proved highly effective in detecting uric acid in human blood serum and urine samples, yielding accurate results with acceptable recovery rates. This dual-mode detection method offers a reliable, sensitive, and cost-effective tool for monitoring uric acid levels, marking a significant advancement in clinical diagnostics and personalized healthcare.

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