ScholarWorks@성균관대학교

초록

Highlights Developed a portable microfluidic device for rapid (4 min) on-site detection of THC-COOH at a low cutoff of 20 ng/mL. Device validation using 100 authentic urine samples showed 100% sensitivity/specificity and high correlation (R2 = 0.9471) with LC-MS/MS. What are the main findings? A test-to-reference (T/R) fluorescence ratio was successfully employed to mitigate urine sample matrix effects and ensure objective, normalized semi-quantitative results. The antibodies used in the cartridge were found to recognize both free THC-COOH and its primary glucuronide-conjugated metabolite, eliminating the need for enzymatic hydrolysis pre-treatment. What are the implications of the main findings? The device provides objective, numerical readouts and digital data management, which overcomes the critical limitations of subjective visual interpretation and poor data integrity found in conventional rapid kits. The system's design enables future integration of creatinine measurement for urine validity testing, suggesting a pathway to a comprehensive on-site device that fully meets forensic toxicology standards.Highlights Developed a portable microfluidic device for rapid (4 min) on-site detection of THC-COOH at a low cutoff of 20 ng/mL. Device validation using 100 authentic urine samples showed 100% sensitivity/specificity and high correlation (R2 = 0.9471) with LC-MS/MS. What are the main findings? A test-to-reference (T/R) fluorescence ratio was successfully employed to mitigate urine sample matrix effects and ensure objective, normalized semi-quantitative results. The antibodies used in the cartridge were found to recognize both free THC-COOH and its primary glucuronide-conjugated metabolite, eliminating the need for enzymatic hydrolysis pre-treatment. What are the implications of the main findings? The device provides objective, numerical readouts and digital data management, which overcomes the critical limitations of subjective visual interpretation and poor data integrity found in conventional rapid kits. The system's design enables future integration of creatinine measurement for urine validity testing, suggesting a pathway to a comprehensive on-site device that fully meets forensic toxicology standards.Highlights Developed a portable microfluidic device for rapid (4 min) on-site detection of THC-COOH at a low cutoff of 20 ng/mL. Device validation using 100 authentic urine samples showed 100% sensitivity/specificity and high correlation (R2 = 0.9471) with LC-MS/MS. What are the main findings? A test-to-reference (T/R) fluorescence ratio was successfully employed to mitigate urine sample matrix effects and ensure objective, normalized semi-quantitative results. The antibodies used in the cartridge were found to recognize both free THC-COOH and its primary glucuronide-conjugated metabolite, eliminating the need for enzymatic hydrolysis pre-treatment. What are the implications of the main findings? The device provides objective, numerical readouts and digital data management, which overcomes the critical limitations of subjective visual interpretation and poor data integrity found in conventional rapid kits. The system's design enables future integration of creatinine measurement for urine validity testing, suggesting a pathway to a comprehensive on-site device that fully meets forensic toxicology standards.Abstract The rapid detection of 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH), a primary cannabis metabolite, is critical for forensic and workplace drug testing. However, conventional immunoassays often lack sensitivity and objectivity. We developed a portable lateral flow immunoassay device with a microfluidic cartridge and fluorescent reader for the semi-quantitative detection of THC-COOH in urine. A test-to-reference fluorescence ratio was employed to mitigate matrix effects and ensure objective results. The device was validated for accuracy, repeatability, and stability using spiked urine samples and compared against validated LC-MS/MS results on 100 authentic urine samples (50 positive and 50 negative). At a cutoff of 20 ng/mL, the device achieved 100% sensitivity and specificity, with repeatability and reproducibility CVs of below 15%. The cutoff index (COI) strongly correlated with LC-MS/MS results (R2 = 0.9471). Crucially, this high correlation with hydrolyzed LC-MS/MS data demonstrates that the antibody recognizes both free and glucuronide-conjugated metabolites, validating its reliability without enzymatic pre-treatment. This microfluidic device enables rapid, sensitive on-site THC-COOH detection, featuring automated data management via Wi-Fi connectivity, enhancing its forensic applicability.

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