ScholarWorks@성균관대학교

초록

A highly accurate and sensitive monitoring platform for wastewater is needed for the detection of wastewater borne pathogens. For the fabrication of an environmental monitoring platform, an aptamer-integrated graphene field-effect transistor (GFET) platform was presented. The detection of pathogenic bacteria living in wastewater is an excellent method for confirming water quality. Here, we developed a target-specific aptamer-loaded GFET for the verification of purified wastewater. Dual graphene channels were micropatterned on the substrate, and each channel was immobilized with an aptamer linked to a chemical linker compound. Pretreated sewage was screened with our newly developed platform, and the detection performance was 101 cfu mL-1 for multidrug-resistant Acinetobacter baumannii and 101 cfu mL-1 for methicillin-resistant Staphylococcus aureus, with a measurement range of 101 to 106 cfu mL-1. Additionally, in the presence of impurities, the platform showed a highly accurate binding affinity between the target and biomolecule without noise caused by interference effects. © 2025 American Chemical Society.

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