ScholarWorks@성균관대학교

초록

The development of ultrasensitive biosensing platforms for carbohydrate antigen 15-3, a crucial serum biomarker for breast cancer monitoring, represents a significant advancement in early cancer diagnostics. This work presents an innovative photoelectrochemical biosensor that synergistically combines the exceptional charge separation properties of an In2S3/ZnIn2S4 type-II heterojunction with the programmable molecular recognition capability of three-way junction (3WJ) DNA nanotechnology. The engineered photoanode architecture demonstrates remarkable photostability and enhanced quantum efficiency, which are further amplified through strategic integration of CdS quantum dots. The unique “on-off-on” detection mechanism of this biosensor arises from the reversible assembly of a 3WJ nanostructure, where target-induced structural reorganization mediates precise photocurrent modulation. This cleverly design achieves excellent analytical performance, spanning four orders of magnitude (0.001–1000 U/mL) with a detection limit of 0.00028 U/mL. Moreover, high selectivity, remarkable reproducibility and long-term stability were confirmed through comprehensive validation studies. This work establishes a new paradigm for molecular diagnostics and opens exciting possibilities for point-of-care cancer screening. © 2025 Elsevier B.V.

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