ScholarWorks@성균관대학교

초록

Biological threats to military and civilian sectors underscore the need for compact, cost-effective, and durable sensor systems capable of the sensitive and selective detection of bio-threat agents. Fluorescence-based techniques, particularly those employing UV light, have proven effective for detecting biological fluorophores such as proteins and cofactors. However, conventional laser-induced fluorescence (LIF) systems, while highly sensitive, are bulky, expensive, and require significant power and maintenance. To address these limitations, light-emitting diodes (LEDs) have emerged as a promising alternative, offering compact, robust, and low-maintenance solutions. Recent advancements in UV LEDs, spanning 200-400 nm, align with the excitation of natural fluorophores found in bacteria, spores, and viruses, enabling the development of practical fluorescence sensors. In this study, we developed a dual-wavelength fluorescence sensor system employing UV LEDs at 280 nm and 365 nm for the selective detection of Bacillus subtilis endospores, a surrogate for the pathogenic Bacillus anthracis. The system integrates optimized optical lenses and a bio-cell utilizing replaceable quartz sample tubes to minimize contamination and enhance usability. The sensor demonstrated selective detection at concentrations as low as 107 spores/mL. This portable, cost-effective system provides a practical solution for rapid and reliable detection of biological threats, meeting critical field requirements for size, weight, and durability.

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