ScholarWorks@성균관대학교

초록

Polymerase chain reaction (PCR) is a critical tool for nucleic acid amplification in molecular diagnosis and genetic analysis. Point-of-care (POC) devices are essential for controlling the spread of infectious diseases, but developing cost-effective chip-based PCR systems remains a challenge. This study introduces a photonic PCR chip featuring a perfect metamaterial absorber made of Ti3C2Tx MXene, silicon dioxide (SiO2), and gold nanoparticles (GNP) in a metal-insulator-metal (MIM) configuration. Fabricated via a solution-processing approach, the absorber demonstrates 98% light absorption without the need for expensive lithographic methods. Utilizing a 940 nm infrared (IR) LED, the chip achieves efficient photothermal effects with heating rates of approximate to 8.3 degrees C s(-)(1) and cooling rates of approximate to 7.2 degrees C s(-)(1) during 30 cycles of lambda-DNA and SARS-CoV-2 amplification, transitioning from 65 degrees C to 95 degrees C. The low cost and high efficiency of the MXene-based metamaterial absorbers highlight their potential as key components for ultrafast, energy-efficient molecular diagnostic chips suitable for on-site applications.

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