ScholarWorks@성균관대학교

초록

In comparison with annihilation-type electrochemiluminescence (ECL), the conventional coreactant-type ECL involving a slow interfacial electron transfer process the exhibits lower ECL efficiency. In this work, an annihilation-type ECL system was developed for the detection of aflatoxin B1 (AFB1), where the prepared highly symmetric metal–organic frameworks act as novel emitters and the endonuclease-driven DNA walker serve as the signal amplifier. A stable and self-luminescent framework (RI-MOF) was constructed through the coordination of carboxylated ruthenium bipyridine ligands with indium (III) cations. In this case, the charge transfer of In3+ to [Ru(H2dcbpy)3]·Cl2 ligand extremely improves the ECL efficiency of RI-MOF under exogenous coreactant-free conditions. The possible annihilation mechanism was investigated by differential pulse voltammetry (DPV) and density-functional theory (DFT) calculations. In addition, DNA walker was employed as high-efficiency signal modulator to enhance the sensitivity of the biosensor. According to the target-triggered signal changes, a wide linear range of 0.01 ng/mL to 100 ng/mL is achieved with a remarkably low detection limit of 3.35 pg/mL. The proposed biosensor is successfully implemented for monitoring the residual level of AFB1 in actual samples, exhibiting the potential application prospect in food safety. © 2025 Elsevier B.V.

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