ScholarWorks@성균관대학교

초록

Ochratoxin A (OTA), a toxic mycotoxin with significant health risks, requires sensitive detection techniques for effective monitoring in both food and environmental settings. By addressing the need for reliable and sensitive analytical methods in food safety, an innovative dual-mode biosensor was developed for detecting OTA in fruit samples. The biosensor leveraged the synergistic effects of coordination-induced electrochemiluminescence (CIECL) from hafnium-based metal-organic frameworks (Hf-MOFs) and the electrochemical (EC) properties of gold‑platinum nanoflowers (Au@Pt NFs). The Hf-MOFs, with their distinctive structure, demonstrated a markedly enhanced ECL emission compared to the monomers and aggregates of 4,4′,4″-nitrilotribenzoic acid (H3NTB). The H3NTB molecule with tertiary nitrogen atoms in exhibited favorable coreactant characteristic, enabling the Hf-MOFs material a self-enhanced intramolecular emission with high ECL relative efficiency. By exploiting the superior differential pulse voltammetry (DPV) response of Au@Pt NFs and their efficient ECL quenching toward Hf-MOFs, ECL signals were successfully generated while minimizing EC signals. This was achieved through an aptamer-induced DNA reaction and a Mg2+-DNAzyme-driven bipedal DNA walker signal amplification strategy. The ultra-sensitive biosensor realized dual-mode detection of OTA with a linear response from 0.01 to 100 ng/mL and detection limits of 2.4 pg/mL for the ECL mode and 6.9 pg/mL for the EC mode. The developed dual-mode biosensing strategy offered a sensitive and accurate tool for OTA detection in fruit samples, holding great potentials for food safety and food quality monitoring. © 2025 Elsevier B.V.

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